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Sigma
Extract-N-Amp™ Plant PCR Kit
sufficient for 1000 extractions, sufficient for 5000 amplifications
Description
| Components | Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix. |
| Other Notes | For additional information, please see www.sigma-aldrich.com/extract-n-amp. |
| Legal Information | Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. |
| Features and Benefits | • Single-step extraction of plant genomic DNA for PCR in less than 15 minutes • No freezing, mechanical disruption, organic extraction, column purification or precipitation required • Specially formulated PCR ReadyMix for use with extract • Hot Start antibody for highly specific PCR amplification of genomic DNA • REDExtract-N-Amp requires no loading buffers or tracking dyes required for gel analysis • Compatible with high-throughput requirements for genetic analysis of plants • Extract stable at 4 °C for at least 6 months |
| General description | The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. |
| Extract-N-Amp is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. | |
| Roche Enzyme Products Conveying Polymerase Patent RightsUse of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as the patented 5′ Nuclease Process claims in US Patents Nos. 5,210,015 and 5,487,972), no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
Properties
| usage | sufficient for 1000 extractions |
| sufficient for 5000 amplifications | |
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
References
| reference | Wang, D., et al., A High-Throughput System for the Rapid Extraction of Plant Genomic DNA for Genome Mapping and Marker-Assisted Breeding Studies J. Assoc. Lab. Autom. 10, 242, (2005) |
